活性氧在金鱼草切花向重力性反应中的作用研究作者姓名:刘华明 范 玉 李韵琦 陈会星 李 茂指导老师姓名:张昭其华南农业大学园艺学院,广州,摘 要向重力性反应是导致金鱼草等切花商品价值降低、包装运输成本高的重要原因之一。而植物的向重力性反应是一个复杂的生理生化过程,因此,研究金鱼草向重力性问题无论在理论上还是在实践上都具有重要意义。有研究表明,活性氧是细胞信号转导和调控组成成分,并且还参与了生长素介导的细胞伸长过程。与此同时,许多研究也表明 Ca+参与了植物向重力性反应的各个环节。本文从这个角度出发,综合考虑这两种重要因素对金鱼草向重力性反应的影响,采用能激发植物内源 HO产生的水杨酸(SA)、过氧化氢酶(CAT)的抑制剂――氨基三唑(AT)、NADPH 氧化酶抑制剂――Imadizole(Im)及一氧化氮释放剂硝普钠(――SNP)处理采后金鱼草切花,研究这些药剂处理对抑制金鱼草向重力性反应中的效果及生理作用,并从生理和分子水平讨论 种主要的抗氧化酶 CAT、APX、NADPH 氧化酶、H+-ATP 酶与活性氧信号及金鱼草向重力性的关系,从分子水平探讨钙信号相关基因 CDPK、CAM 与向重力性反应的关系。关键词:活性氧 抑制剂 诱导剂 金鱼草 向重力性AbstractThe Regulating Root Gravitropism has bad effects on cut flower, Antirrhinum majus for instance—lower value and high cost of packaging and transportation It’s a complex physiological and biochemical process So it’s significant to study the Gravitropism in theory and practice Some studies showed that Reactive oxygen controlled to some degree the regulation in the Gravitropism of root What’s more, it is component of cellular signal transduction and controlling,involved in auxin-mediated cell elongation Research also shows that the Ca + are involved in the Gravitropism of root of the various links From this perspective, we use medicaments of SA,CAT,AT,IM,SNP that can stimulate plants to produce HO to handle Post-harvest Antirrhinum majus, research the effects and physiological function of medicaments processing on Regulating Root Gravitropism and discuss the connection between enzyme of CAT,APX,NADPH, H + - ATP and signal of Reactive oxygen species and the Gravitropism at the physiological and molecular level and the relation of the Ca signal gene of CDPK、CAM and the Gravitropism at molecular levelKey words:Reactive Oxygen;Inhibitor; Inducer;Antirrhinum majus;Gravitropism目 录 前言 材料与方法 材料及处理 药剂浓度筛选 方法与步骤 经药剂处理后切花形态特征的观察 生理指标的测定 APX